U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    Hyal5 hyaluronoglucosaminidase 5 [ Mus musculus (house mouse) ]

    Gene ID: 74468, updated on 3-Apr-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    Hyal5provided by MGI
    Official Full Name
    hyaluronoglucosaminidase 5provided by MGI
    Primary source
    MGI:MGI:1921718
    See related
    Ensembl:ENSMUSG00000029678 AllianceGenome:MGI:1921718
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    4933439A12Rik
    Summary
    Enables hyalurononglucosaminidase activity. Acts upstream of or within penetration of zona pellucida. Located in acrosomal vesicle and external side of plasma membrane. Orthologous to human SPAM1 (sperm adhesion molecule 1). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Restricted expression toward testis adult (RPKM 24.7) See more
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Location:
    6 A3.1; 6 11.28 cM
    Exon count:
    5
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (24857982..24891963)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (24857937..24891959)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_15361 Neighboring gene sperm adhesion molecule 1 Neighboring gene sperm adhesion molecule 1 pseudogene Neighboring gene predicted gene, 22267 Neighboring gene STARR-seq mESC enhancer starr_15363 Neighboring gene STARR-seq mESC enhancer starr_15364 Neighboring gene STARR-seq mESC enhancer starr_15365 Neighboring gene transmembrane protein 229A

    Genomic regions, transcripts, and products

    Go to the top of the page Help

    Go to nucleotide: Graphics FASTA GenBank

    Expression

    Go to the top of the page Help
    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect. Park S, et al. FASEB J, 2019 Dec. PMID 31670981
    2. Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? Kang W, et al. J Reprod Dev, 2010 Feb. PMID 19926939
    3. Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization. Kimura M, et al. Biol Reprod, 2009 Nov. PMID 19605784
    4. Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan. Reitinger S, et al. Biochem J, 2007 Jan 1. PMID 16925524, Free PMC Article
    5. Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass. Kim E, et al. Proc Natl Acad Sci U S A, 2005 Dec 13. PMID 16330764, Free PMC Article

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A comparative analysis between WT and Spam1/Hyal5 dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the cumulus-oocyte complex.
      Title: SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect.
    2. The data from this paper suggests that the hyaluronan-degrading activity of the sperm hyaluronidase HYAL5 may not be required for fertilization, at least in the mouse.
      Title: Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?
    3. The HYAL5-deficient female mice were fully fertile and yielded normal litter sizes. HYAL5-deficient epididymal sperm is functionally normal. Thus, HYAL5 may be dispensable for fertilization.
      Title: Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization.
    4. Mouse SPAM1 and HYAL5 but not HYALP1 proteins exhibit hyaluronidase activity at neutral pH.
      Title: Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan.
    Submit: New GeneRIF Correction

    Variation

    Go to the top of the page Help

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

    Go to the top of the page

    General gene information

    Go to the top of the page Help

    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables hyalurononglucosaminidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables hyalurononglucosaminidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fusion of sperm to egg plasma membrane involved in single fertilization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in hyaluronan catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within penetration of zona pellucida IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in acrosomal vesicle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in acrosomal vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasmic vesicle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in external side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in side of membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    hyaluronidase-5
    Names
    hyal-5
    hyaluronidase Hyal-5
    hyaluronidase PH-20
    NP_001404776.1
    NP_001404777.1
    NP_001404778.1
    NP_083233.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001417847.1 → NP_001404776.1  hyaluronidase-5 isoform a precursor

      Status: VALIDATED

      Source sequence(s)
      AC127559
      UniProtKB/Swiss-Prot
      Q812F3
      Related
      ENSMUSP00000031689.6, ENSMUST00000031689.6

    2. NM_001417848.1 → NP_001404777.1  hyaluronidase-5 isoform b

      Status: VALIDATED

      Source sequence(s)
      AC127559

    3. NM_001417849.1 → NP_001404778.1  hyaluronidase-5 isoform c

      Status: VALIDATED

      Source sequence(s)
      AC127559

    4. NM_028957.3 → NP_083233.1  hyaluronidase-5 isoform a precursor

      See identical proteins and their annotated locations for NP_083233.1

      Status: VALIDATED

      Source sequence(s)
      AC127559
      Consensus CDS
      CCDS19948.1
      UniProtKB/Swiss-Prot
      Q812F3
      UniProtKB/TrEMBL
      Q9CUA5
      Related
      ENSMUSP00000144011.2, ENSMUST00000200968.4
      Conserved Domains (1) summary
      pfam01630
      Location:42 → 369
      Glyco_hydro_56; Hyaluronidase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      24857982..24891963
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q812F3.1 GenPept UniProtKB/Swiss-Prot:Q812F3

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous