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Sample GSM862723 Query DataSets for GSM862723
Status Public on Apr 10, 2012
Title RenLab-HiC-hESC, replicate one
Sample type SRA
Source name Human ES
Organism Homo sapiens
Characteristics cell line: hESC line H1
restriction enzyme: HindIII
Treatment protocol None
Growth protocol mESC line J1 was maintained on mitomycin C-inactivated MEF feeder layers in DME containing 15% fetal calf serum, leukemia inhibiting factor, penicillin/streptomycin, L-glutamine and non-essential amino acids. All ES cells were passaged on 0.2 % gelatin twice to deplete feeder cells before harvest for experiments. H1 and IMR90 cells were grown as described in: Hawkins, R.D. et al. Distinct epigenomic landscapes of pluripotent and lineage-committed human cells. Cell Stem Cell 6, 479-91
Extracted molecule genomic DNA
Extraction protocol Hi-C experiment were conducted in biological replicates using HindIII according to previous publication (Lieberman-Aiden, E. et al. Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science 326, 289-93 (2009).).
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina HiSeq 2000
Data processing fastq: Illumina's HiSeq Control Software
hic.summary.txt:The paired-end Hi-C reads were mapped to mouse genome build mm9 and human genome hg18 using an in-house pipeline based on BWA using default parameter. BWA SAMPE was used to merged the pairs. Duplicated reads from the same biological library were removed.
Genome Build:
hESC_HindIII_HiC.nodup.summary.txt: hg18
Submission date Jan 17, 2012
Last update date May 15, 2019
Contact name Feng Yue
Organization name Ludwig Institue for Cancer Research
Street address 9500 Gilman Dr.
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platform ID GPL11154
Series (2)
GSE35156 Conserved Topological Domains in Mammalian Genomes Identified by High-resolution Analysis of Chromatin Interactions
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
SRA SRX116344
BioSample SAMN00773754

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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