|
| Status |
Public on Jan 26, 2012 |
| Title |
46T_059_01 IgM |
| Sample type |
protein |
| |
|
| Source name |
Human antiserum
|
| Organism |
Homo sapiens |
| Characteristics |
sample group: SLE Histone positive
|
| Extracted molecule |
protein |
| Extraction protocol |
Whole antiserum was obtained.
|
| Label |
DyLight-649-conjugated goat anti-human IgM antibody
|
| Label protocol |
Commercially available DyLight 649-conjugated goat anti-mouse or goat anti-human antibody was added to the microarray at 1:500 in 10% fetal bovine serum in phosphate-buffered saline with 0.1% Tween-20.
|
| |
|
| Hybridization protocol |
Human antiserum was probed on the HEMP array at 1:250 dilution in 10% fetal bovine serum with 50 mM Tris pH 7.5, 150 mM sodium chloride and 0.05% NP-40, and incubated overnight at 4 degrees Celsius.
|
| Scan protocol |
Fluorescent array images were collected at 635 nm with a GenePix 4000B fluorescent scanner at 10 micron resolution and image intensity data were extracted and analyzed with GenePix Pro 6.1 analysis software.
|
| Description |
46T
Human antiserum was obtained from blood collected from a SLE Histone positive patient. A 1:250 dilution of this antiserum was then applied onto the HEMP array V1.0, labeled with a goat anti-human IgM secondary antibody conjugated to DyLight-649 (Jackson ImmunoResearch), and scanned with a GenePix 4000B scanner using GenePix Pro 6.1.
|
| Data processing |
Data were background corrected and flagged values were removed. The remaining replicate values were averaged.
|
| |
|
| Submission date |
Oct 01, 2011 |
| Last update date |
Jan 26, 2012 |
| Contact name |
Chih Long Liu |
| Organization name |
Stanford University
|
| Department |
Medicine - Imm. & Rheum
|
| Lab |
Utz
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL14644 |
| Series (2) |
| GSE32543 |
Human and murine serum reactivity to specific histone posttranslational modifications in neutrophil extracellular traps |
| GSE32544 |
Specific post-translational histone mod. of neutrophil extracellular traps as immunogens & potential SLE Ab targets. |
|
