|
| Status |
Public on Oct 28, 2011 |
| Title |
Adenocarcinoma_sample_61A |
| Sample type |
RNA |
| |
|
| Source name |
Non-small cell lung cancer tissue, short-term survival, adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: non-small cell lung cancer
histology: adenocarcinoma
survival: short
registry year: 2003
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 2-10 frozen tissue sections (10mm) using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and the RNeasy Mini Kit (Qiagen), respectively.
|
| Label |
biotin
|
| Label protocol |
Sample preparation was performed according to the GeneChip® Expression Analysis Technical Manual (Affymetrix Inc., Rev. 5).
|
| |
|
| Hybridization protocol |
Sample hybridization was performed according to the GeneChip® Expression Analysis Technical Manual (Affymetrix Inc., Rev. 5).
|
| Scan protocol |
The arrays were then washed using Affymetrix fluidics stations, and scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
61A_081029
Hybridized to HG-U133 Plus 2.0
|
| Data processing |
Analyses of the gene expression data were carried out in the freely available statistical computing language R (http://www.r-project.org) using packages available from the Bioconductor project. The raw data was normalized using the robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Apr 12, 2011 |
| Last update date |
Oct 28, 2011 |
| Contact name |
Anders Isaksson |
| E-mail(s) |
anders.isaksson@medsci.uu.se
|
| Organization name |
Uppsala University
|
| Street address |
Akademiska sjukhuset ing61 3tr
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE28571 |
Gene Copy Number Aberrations are Associated with Survival in Histological Subgroups of Non-Small Cell Lung Cancer (expression data) |
| GSE28582 |
Gene Copy Number Aberrations are Associated with Survival in Histological Subgroups of Non-Small Cell Lung Cancer |
|
