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Status |
Public on Aug 31, 2015 |
Title |
Sk Mus_GW501516_10 mg/kg_Day15_Rep1 |
Sample type |
RNA |
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|
Source name |
Sk Mus_GW501516_10 mg/kg_Day15
|
Organism |
Mus musculus |
Characteristics |
tissue: Skeletal Muscle compound: GW501516 dose: 10 mg/kg timepoint: 15 days
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen miRNEasy was used according to maufacturers instruction including DNAse I treatment.
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the Nugen protocol from 100ng total RNA using Nugen WT Ovation V2 (cat. 3100-60) and Nugen Encore Biotin (cat. 4200-60)
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Hybridization protocol |
Following fragmentation, 3.75ug of cDNA were hybridized for 18 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
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Description |
Sk Mus from mouse treated with GW501516 at a dose of 10 mg/kg for 15 days (Mouse_106_Sk Mus)
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Data processing |
The data were analyzed with Robust Multi-chip Average (RMA) normailzation method using Partek (Liver, heart, kidney, and skeletal muscle samples were each normalized independently)
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Submission date |
Mar 15, 2011 |
Last update date |
Aug 31, 2015 |
Contact name |
Runxuan Zhang |
E-mail(s) |
runxuan.zhang@hutton.ac.uk
|
Phone |
+441382862663
|
Organization name |
The James Hutton Institute
|
Department |
Information and Computational Sciences
|
Street address |
Errol Road, Invergowrie
|
City |
Dundee |
State/province |
Angus |
ZIP/Postal code |
DD2 5DA |
Country |
United Kingdom |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE27948 |
Pharmacology of PPAR isoform agonists on mouse |
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