|
| Status |
Public on Feb 23, 2011 |
| Title |
BMAL1_ZT22_ChIP-Seq_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Liver cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Liver
time: ZT22
chip antibody: BMAL1
|
| Growth protocol |
Mice were housed under a 12-h light/12-h dark regimen with food and water available ad libitum. ZT0 is defined as the time when the lights are turned on. Animals were housed for 3 weeks under the indicated photoperiods.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Crosslinked nuclei from liver cells were sonicated and protein-DNA complexes were isolated with a BMAL1 antibody (non-commercial). Libraries were prepared according to Illumina's instructions.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Chromatin IP against BMAL1
sample 6a
|
| Data processing |
Alignment: Sequence reads were aligned to the mouse genome (mm9) using Bowtie software
bedgraph: each read was shifted by +/- 75 bp for plus and minus strand respectively and then normalized by the total number of aligned reads in the library.
|
| |
|
| Submission date |
Jan 13, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Guillaume Rey |
| E-mail(s) |
guillaume.rey@unige.ch
|
| Organization name |
University of Geneva
|
| Street address |
Campus Biotech, Chemin des Mines 9
|
| City |
Geneva |
| ZIP/Postal code |
1202 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL9185 |
| Series (1) |
| GSE26602 |
Genome-Wide And Phase-Specific DNA-Binding Rhythms Of BMAL1 Control Circadian Output Functions In Mouse Liver |
|
| Relations |
| SRA |
SRX038442 |
| BioSample |
SAMN00190982 |
