|
| Status |
Public on Jun 16, 2010 |
| Title |
Run4.aUa.010110d.SkMDbt1147D.CEL-DB stimulate |
| Sample type |
RNA |
| |
|
| Source name |
human skeletal muscle from diabetic individuals after insulin therapy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skeletal muscle
status: diabetic
agent: insulin
|
| Treatment protocol |
regular insulin (Humulin, Eli Lily & Co., Indianapolis, IN) at a constant rate of 200 mU/m2/min
|
| Growth protocol |
human skeletal muscle collected from 20 insulin sensitive, 20 insulin resistant, and 15 diabetic individuals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Ultraspec RNA isolation Kit from Biotecx Laboratories, Inc according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
The labeled cRNA were hybridized for 16 h at 45C on Affymetrix Hu95A chip. Affymetrix chips were washed and then stained with a streptavidin-phycoerythrin conjugate.
|
| Scan protocol |
The arrays were scanned at a resolution of 3mm, using a specifically design confocal scanner (Affymetrix).
|
| Data processing |
The data were normalized by using quantile normalization and then obtained the probe set intensity using RMA or MAS5 as implemented in the affy package in Bioconductor. The present/absent calls for each probe set were generated based on Wilcoxon signed rank test using the mass5call function in the affy package.
|
| |
|
| Submission date |
Jun 11, 2010 |
| Last update date |
Mar 03, 2011 |
| Contact name |
Jelai Wang |
| E-mail(s) |
jwang@ms.soph.uab.edu
|
| URL |
http://www.ssg.uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Biostatistics
|
| Street address |
1665 University Blvd, Ryals 327
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL91 |
| Series (1) |
| GSE22309 |
Expression data from human skeletal muscle |
|
