|
| Status |
Public on Jun 30, 2011 |
| Title |
Yeast_met30-6_35°C_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
S. cerevisiae, met30-6, 35°C
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: met30-6 (py283)
|
| Treatment protocol |
Following 1 hour incubation at either permissive (RT) or non-permissive (35°C) temperature, cells were harvested and flash frozen in liquid nitrogen.
|
| Growth protocol |
Yeast cells were grown to mid-log phase (0.5–2 x 107 cells/ml) at 25°C (RT), 300 rpm, in YPDA medium before selected samples were subjected to heat-inactivation for 1 hour at 35°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RiboPure™-Yeast kit (Ambion) and RNA quality was checked using Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
Biotin
|
| Label protocol |
Biotin-labeled cRNA probes were generated from total RNA using the One-Cycle Target Labeling Assay (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization of labeled cRNA probes to Affymetrix GeneChip Yeast Genome 2.0 arrays (www.affymetrix.com) was done according to manufacturer’s specifications.
|
| Scan protocol |
The arrays were scanned using Affymetrix GeneChip Scanner 3000 and raw data generated using the GeneChip Operating System GCOSv1.4.
|
| Description |
growth in YPDA media
|
| Data processing |
Gene expression data were normalized in dChip (www.dchip.org). Model-based expression indices (MBEIs) were calculated and log2 transformed. We have only shown the data pertaining to the Saccharomyces cerevisiae probes and had deleted the data pertaining to Saccharomyces pombe since that data is irrelevant to our study, which only uses S. cerevisiae. We do show ALL Saccharomyces cerevisiae probe data.
|
| |
|
| Submission date |
Jun 10, 2010 |
| Last update date |
Jun 30, 2011 |
| Contact name |
Mariam Aghajan |
| Organization name |
UCLA
|
| Street address |
650 Charles E. Young Drive South, CHS 23-231
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (2) |
| GSE22270 |
Expression data from Saccharomyces cerevisiae Met30 temperature-sensitive strain |
| GSE22272 |
Chemical Genetics of TOR Identifies an SCF Family E3 Ubiquitin Ligase Inhibitor |
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