|
| Status |
Public on Feb 03, 2010 |
| Title |
daf-2(e1368) xbp-1 vs daf-2(e1368)_2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
daf-2(e1368) xbp-1(zc12) day1
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genome/variation: daf-2(e1368) xbp-1(zc12)
developmental stage: day 1
|
| Growth protocol |
Eggs were synchronized by bleaching, grown at 20 degrees and harvested as day-1 adults (after 72 hours for e1368 mutants, after 96 hours for e1370 mutants).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Arrays labelled using the Agilent QuickAmp Kit according to manufacturer's instructions. Protocols available from www.agilent.com.
|
| |
|
| Channel 2 |
| Source name |
daf-2(e1368)
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genome/variation: daf-2(e1368)
developmental stage: day 1
|
| Growth protocol |
Eggs were synchronized by bleaching, grown at 20 degrees and harvested as day-1 adults (after 72 hours for e1368 mutants, after 96 hours for e1370 mutants).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Arrays labelled using the Agilent QuickAmp Kit according to manufacturer's instructions. Protocols available from www.agilent.com.
|
| |
|
| |
| Hybridization protocol |
Arrays were hybridised using the Hi-RPM hybridization kit according to manufacturer's instructions. Protocols available from www.agilent.com.
|
| Scan protocol |
Scanning was performed on G2565BA DNA Scanner and feature extraction was made by FE software Version 9.5 (Agilent) using extraction protocol GE2-v5_95_Feb07.
|
| Description |
total RNA from day 1 animals
|
| Data processing |
Lowess normalization and analysis was performed in GeneSpring (Agilent Technologies). Arrays were first filtered to remove features which were at or below average background signal.
|
| |
|
| Submission date |
Feb 02, 2010 |
| Last update date |
Feb 02, 2010 |
| Contact name |
sivan korenblit |
| E-mail(s) |
sivankorenblit@gmail.com
|
| Organization name |
Bar-Ilan University
|
| Department |
life sciences
|
| Lab |
Korenblit
|
| Street address |
Ramat Gan
|
| City |
Ramat Gan |
| ZIP/Postal code |
52900 |
| Country |
Israel |
| |
|
| Platform ID |
GPL8209 |
| Series (1) |
| GSE20148 |
C. elegans microarrays: daf-2(-) vs. daf-2(-) xbp-1(-) |
|
