|
| Status |
Public on Feb 09, 2022 |
| Title |
NHP_459s_B10_S34_L006_ |
| Sample type |
SRA |
| |
|
| Source name |
NHP cells
|
| Organism |
Macaca fascicularis |
| Characteristics |
cell type: left ventricle heart slices
cell line: NHP cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells within chimeric heart slices were isolated by dissociation using 20 U/ml papain (Worthington Biochemical, USA). Single cells were collected using FACSAria III (Becton Dickinson) into individual wells of 384-well plates containing Smart-Seq2 cell lysis buffer.
RNAseq libraries were prepared using compatible Tn5 and Nextera index primers (Illumina, USA) after complementary deoxyribonucleic acid (cDNA) synthesis using Smart-Seq2 protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
The Genome Analyzer Analysis Pipeline (Illumina, USA) was used to process the sequencing files of raw reads in the FASTQ format
Sequenced reads were trimmed for low-quality using Trimmomatic (default parameters)
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using tophat2 (default parameters)
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include HTSeq-count values for each Sample
|
| |
|
| Submission date |
Jun 25, 2020 |
| Last update date |
Feb 09, 2022 |
| Contact name |
Gianluca Santamaria |
| E-mail(s) |
santamariagianluca@tum.de
|
| Organization name |
Klinikum rechts der Isar – Technical University Munich
|
| Department |
Department of Medicine I
|
| Lab |
Molecular Cardiology
|
| Street address |
Ismaninger Strasse 22
|
| City |
Munich |
| State/province |
Germany |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20302 |
| Series (1) |
| GSE153282 |
Migratory and anti-fibrotic programmes define the regenerative potential of human cardiac progenitors |
|
| Relations |
| BioSample |
SAMN15373664 |
| SRA |
SRX8620311 |
