|
| Status |
Public on Feb 05, 2009 |
| Title |
Line 20b 3_Experiment 1, 430B |
| Sample type |
RNA |
| |
|
| Source name |
quadriceps muscle
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: CUGexp transgenic Line 20b
Gender: male
Age: 6 months old
|
| Treatment protocol |
No treatments other than constitutive genetic modifications
|
| Growth protocol |
Mice were reared under normal conditions. CUGexp mice were sacrificed at 6 months of age. Mbnl1-null and Clcn1-null mice were sacrificed at 3 months of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted into Tri-Reagent as recommended by supplier.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with kits from Enzo as recommended by supplier.
|
| |
|
| Hybridization protocol |
Hybridization, washes, and staining with phycoerythrin-streptavidin (including antibody enahncement step) were carried out exactly as recommended by Affymetrix for eukaryotic expression arrays, using hybridization oven and fluidics station purchased from Affymetrix.
|
| Scan protocol |
Scans were done with Affymetrix scanner exactly as recommended by Affymetrix.
|
| Description |
Line 20b 3_Experiment 1, 430B
|
| Data processing |
background subtraction and normalization were done with the GCRMA method.
|
| |
|
| Submission date |
Feb 03, 2009 |
| Last update date |
Feb 04, 2009 |
| Contact name |
Stephen Welle |
| E-mail(s) |
swelle@rochester.rr.com
|
| Organization name |
University of Rochester
|
| Street address |
601 Elmwood Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE14691 |
Transcriptional and post-transcriptional impact of toxic RNA in myotonic dystrophy |
|
