|
| Status |
Public on Sep 09, 2009 |
| Title |
genotype: Incrocio Manzoni - disease type: Bois Noir infected - rep2 |
| Sample type |
RNA |
| |
|
| Source name |
leaf vein (genotype: Incrocio Manzoni - disease type: Bois Noir infected)
|
| Organism |
Vitis vinifera |
| Characteristics |
Cultivar Incrocio Manzoni 60.13 (Riesling Renano x Pinot Bianco) (Germplasm Name); PO:0020138 leaf vein, (Organism Part); PO:0009012 plant growth and development stages (Development Stage); field soil (Growth Media).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tris-LiCl
|
| Label |
biotin
|
| Label protocol |
Affy-Eukaryotic Target Preparation
|
| |
|
| Hybridization protocol |
Affy- Eukaryotic Target Hybridization
|
| Scan protocol |
Affy- Array Scanning
|
| Description |
Field-grown Incrocio Manzoni plant naturally infected with Bois Noir phytoplasma
|
| Data processing |
The MAS5.0 Normalization is performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org). The target value is 500 for all cases. The code was developed using R2.4.1, Bioconductor 1.9, and simpleaffy 2.8.0. The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html. The normalization scripts are available from the PLEXdb team by request.
|
| |
|
| Submission date |
Sep 18, 2008 |
| Last update date |
Sep 09, 2009 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL1320 |
| Series (1) |
| GSE12842 |
Gene expression in grapevine in response to Bois noir infection |
|
