|
| Status |
Public on Aug 26, 2018 |
| Title |
NHP_G_DPI_-13 |
| Sample type |
SRA |
| |
|
| Source name |
Whole Blood
|
| Organism |
Macaca mulatta |
| Characteristics |
dpi: -13
|
| Treatment protocol |
Samples were inactivated with Trizol LS
|
| Growth protocol |
Whole blood samples were collected from NHPs at various days post Ebola Makona infection and treated with Trizol LS at a ratio of 1:3 of blood to Trizol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extration was done following the standard Trizol protocol
Libraries were contstructed using the Illumina Ribo Zero prep kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Ebola Makona infected Non-Human Primate
|
| Data processing |
Raw files were filtered for read quality and length using the fastx-toolkit
Alignments were generated using Tophat which calls Bowtie2
Counts were generated with HTSeq-count
Genome_build: Rhesus Macaque
Supplementary_files_format_and_content: Count files as a tab delimiated text file
|
| |
|
| Submission date |
Jun 13, 2018 |
| Last update date |
Aug 26, 2018 |
| Contact name |
Emily Speranza |
| E-mail(s) |
speranzaee@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
NIAID
|
| Lab |
Laboratory of Immune System Biology
|
| Street address |
4 MEMORIAL DR
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21120 |
| Series (1) |
| GSE115785 |
Whole blood RNA-Sequencing from 12 Rhesus Macaques following Ebola Makona Infection |
|
| Relations |
| BioSample |
SAMN09423405 |
| SRA |
SRX4212996 |
