|
| Status |
Public on Nov 29, 2018 |
| Title |
NveB.3 |
| Sample type |
SRA |
| |
|
| Source name |
Naive B Cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Naive B Cell
cell lineage: B Cell Lineage
antibodies: CD3:- CD19:+ CD27:Lo
tissue: Peripheral Blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Buffy pack from adult donors
PBMCs were isolated with Ficoll Density Gradient
RNA was isolated from two biological repliates (RNAeasy plus micro kit, QIagen)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Haemopedia-Human-RNASeq_raw.txt
Haemopedia-Human-RNASeq_rpkm.txt
|
| Data processing |
Sequence reads were alligned using the R Subread aligner
Genewise counts were obtained using FeatureCounts
Counts were converted to counts per million mapped reads per kilobase of transcript
Genome_build: GRCh38/hg38
Supplementary_files_format_and_content: raw_counts file is the counts as given by FeatureCounts
Supplementary_files_format_and_content: norm_Log2RPKM files give the normalized Log2 reads per kilobase of transcript, per million mapped reads
|
| |
|
| Submission date |
Jun 13, 2018 |
| Last update date |
Nov 29, 2018 |
| Contact name |
Carolyn A de Graaf |
| E-mail(s) |
degraaf@wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE115736 |
Haemopedia: Human Haematopoietic Gene Expression |
|
| Relations |
| BioSample |
SAMN09405289 |
| SRA |
SRX4203032 |
