|
| Status |
Public on Apr 12, 2018 |
| Title |
NHP_4_DPI_21 |
| Sample type |
SRA |
| |
|
| Source name |
Whole Blood
|
| Organism |
Macaca fascicularis |
| Characteristics |
tissue: Whole Blood
agent: Ebola virus Makona
route: IN Pipette
dpi: 21
|
| Treatment protocol |
12 Cynomolgus Macaques infected with EBOV-Makona through intranasal routes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
500ul whole blood mixed with 500 ul of TRIzol LS and purified using the PureLink RNA MiniKit
Constructed on a Sciclone G3 Liquid Handling Robot with TruSeq Stranded Total RNA Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequenced on a HiSeq 2500 with paired-end 100bp reads in dual-index format
Reads were filtered with FASTX Toolkit v0.0.14
Aligned to Genome using Bowtie 2 v2.2.6 and Tophat v2.1.0
Count Tables Generated using HTSeq v0.5.4 and Python v.2.7.3
Genome_build: Cynomolgus Macques Genome v5.0
Supplementary_files_format_and_content: Text files with raw count values from HTSeq
|
| |
|
| Submission date |
Sep 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Emily Speranza |
| E-mail(s) |
speranzaee@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
NIAID
|
| Lab |
Laboratory of Immune System Biology
|
| Street address |
4 MEMORIAL DR
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL20302 |
| Series (1) |
| GSE103825 |
A conserved transcriptional response to intranasal Ebola virus exposure in nonhuman primates before onset of fever |
|
| Relations |
| BioSample |
SAMN07638702 |
| SRA |
SRX3182610 |
