|
| Status |
Public on Sep 01, 2017 |
| Title |
CT26 cells treated with DMSO |
| Sample type |
RNA |
| |
|
| Source name |
cells from culture
|
| Organism |
Mus musculus |
| Characteristics |
cell line background: CT26
treated with: DMSO for 24h
|
| Treatment protocol |
CT26 cells were treated with DMSO or Senexin B for 24 h.
|
| Growth protocol |
CT26 cells were maintained in RPMI-1640 (ThermoFisher Scientific) with 10% fetal bovine serum (FBS) (Atlanta Biologics), 1% penicillin-streptomycin and 2 mM L-glutamine (VWR)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cells in p-6 well plate with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip MoGene-2_0-st Arrays (Affymetrix) and scanned at Functional Genomics Core of the Center for Targeted Therapeutics at the University of South Carolina.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
RMA expression value derived from Expression Console software; core-exon analysis
affymetrix-algorithm-name = rma-gene-full
affymetrix-algorithm-version = 1.0
affymetrix-array-type = MoGene-2_0-st
program-name = Expression Console
program-version = 1.3.1.187
|
| |
|
| Submission date |
Aug 31, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Mengqian Chen |
| E-mail(s) |
chenm@cop.sc.edu
|
| Organization name |
University of South Carolina
|
| Department |
College of Pharmacy
|
| Street address |
CLS710, 715 Sumter Street
|
| City |
Columbia |
| State/province |
SC |
| ZIP/Postal code |
29208 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE103346 |
CDK8 Mediator kinase drives colon cancer hepatic metastasis by regulating gene expression of the matrix metalloproteinase network [Affymetrix] |
| GSE104970 |
CDK8 Mediator kinase drives colon cancer hepatic metastasis by regulating gene expression of the matrix metalloproteinase network |
|
