Wild type and Tao3−/− RAW264.7 cells were infected with Vesicular Stomatitis Virus (VSV, MOI=1) or herpes simplex virus 1 (HSV-1, MOI=5) for 6h.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from the cells using the TRIZOL method (InVitrogen), followed by digestion with RNase-free DNase (Qiagen) and purification on RNeasy spin columns using RNeasy Minikit (Qiagen).
Label
Biotin
Label protocol
Total RNA were amplified, labeled and purified by using GeneChip 3’ IVT Express Kit (Cat #901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA at the Shanghai Biotechnology Corporation (Shanghai, China).
Hybridization protocol
The hybridization was done at the Shanghai Biotechnology Corporation (Shanghai, China). Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat #900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat #00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat #00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Scan protocol
The scanning was done at the Shanghai Biotechnology Corporation (Shanghai, China). Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description
Gene expression data from wild type RAW264.7 cells infected with VSV
Data processing
Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). The genes in each Matrix template are arranged according to affymetrix probe set id.
