|
| Status |
Public on Oct 05, 2016 |
| Title |
AR G2/M ChIP-Seq |
| Sample type |
SRA |
| |
|
| Source name |
LNCaP Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
cell cycle arrest treatment: 2μg/mL aphidicolin for 15 hours, followed by washout and treatment with 50ng/mL nocodozole for 9 hours +3 hour 10nm DHT
dht treatment: +
chip antibody: AR N-20 (SC-816)
|
| Treatment protocol |
Cells were treated with indicated drug 24 hours prior to harvest, and 10nM DHT 3 hours prior to harvest, with the exception of G0 control which was treated with CDT for 72 hours and EtOH 3 hours prior to harvest
|
| Growth protocol |
LNCaP cells were cultured in hormone proficient media unless otherwise indicated.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP was performed as previously described by Schmidt et al 2009
Illumina library prep kit was utilyzed according to manufacturer's instructions
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
alignment to hg18 genome with bowtie
peak calling with MACS1.4 with a 10E-8 p-value cutoff
Genome_build: hg18
Supplementary_files_format_and_content: BED file with called peaks
|
| |
|
| Submission date |
Jun 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher McNair |
| E-mail(s) |
christopher.mcnair@jefferson.edu
|
| Organization name |
Thomas Jefferson University
|
| Department |
Medical Oncology
|
| Street address |
833 Chestnut Street Ste 1140
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE82202 |
Identification of cell cycle regulated, DHT induced AR binding events |
| GSE82203 |
Identification of DHT induced cell cycle dependent trascriptome and AR binding events |
|
| Relations |
| BioSample |
SAMN05199936 |
| SRA |
SRX1818077 |
