|
| Status |
Public on Mar 29, 2016 |
| Title |
Mutant, biological rep37 |
| Sample type |
RNA |
| |
|
| Source name |
DG of adult mutant mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Dentate gyrus
genotype: Mutant
age: 22w
backgrond: C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and cleanup byRNeasy Micro kit (QIAGEN) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 (GCS3000)
|
| Description |
Gene expression data from the DG of mutant mouse (24-h LA: 54865.2)
|
| Data processing |
The data were analyzed with Affymetrix Expression Cosole (version 1.1). The normalization used in RMA is quantile normalization.
|
| |
|
| Submission date |
Apr 27, 2015 |
| Last update date |
Mar 29, 2016 |
| Contact name |
Hideo Hagihara |
| E-mail(s) |
h-hagi@fujita-hu.ac.jp
|
| Phone |
81-562-93-9383
|
| Organization name |
Fujita Health University
|
| Department |
Institute for Comprehensive Medical Science
|
| Street address |
1-98 Dengakugakubo, Kutsukake-cho
|
| City |
Toyoake |
| ZIP/Postal code |
4701192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE68293 |
Gene expression microarray analysis on the dentate gyrus of alpha-CaMKII HKO mice |
|
