cell line: DETROIT562 tissue: Pharynx disease: carcinoma from pleural effusion
Extracted molecule
total RNA
Extraction protocol
RNA was extracted from cell lines using the Qiagen RNeasy kit as per the manufacturer's instructions. RNA quantity was determined using a Nanodrop spectrophotometer and both 260/280 and 260/230 ratios were evaluated. RNA quality was assessed using an Agilent RNA 6000 Nano Chip and the RNA Integrity Number (RIN) value of each sample was determined.
Label
biotin
Label protocol
Affymetrix protocol
Hybridization protocol
Affymetrix protocol
Scan protocol
Affymetrix protocol
Description
Untreated
Data processing
The data were analysed with the robust multichip analysis algorithm. The data is returned in log2 normalised format. The RMA version used was from the bioconductor package affy, stable release version number 1.40.
