|
| Status |
Public on Aug 09, 2007 |
| Title |
Testis_wild type_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
testis
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: wild type
Strain: C57BL/6J
Age: 8-weeks
|
| Treatment protocol |
Testis was snap-frozen and stored at -80°C.
|
| Growth protocol |
male mice were sacrificed at 8 weeks old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by Qiagen RNEasy clean-up.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using using the GCS3000 scanner..
|
| Description |
Gene expression data from wild type testis, biological rep2.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 11, 2006 |
| Last update date |
Aug 11, 2006 |
| Contact name |
Jeremy Wang |
| Phone |
215-746-0160
|
| Fax |
215-573-5188
|
| Organization name |
University of Pennsylvania
|
| Department |
Animal Biology
|
| Street address |
3800 Spruce Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5510 |
Expression data from wild type and Taf7l mutant testes |
|
