|
| Status |
Public on Dec 22, 2013 |
| Title |
MB003 |
| Sample type |
RNA |
| |
|
| Source name |
Primary NSCLC
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: M
histology: adenocarcinoma
t-stage: 2
n-stage: 0
m-stage: 0
Stage: 1B
age: 69.15
smoking: Current
survival time: 7.32
status: alive
disease-free survival time: 7.324
recurrence: N
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
Microarray data for 181 samples was preprocessed using RMA implemented in R/Bioconductor ‘affy’ package (ref: PMID:12538238). Normalized data values were transformed in log2 space and used in subsequent analysis
|
| |
|
| Submission date |
Aug 21, 2013 |
| Last update date |
Dec 22, 2013 |
| Contact name |
Ming Tsao |
| E-mail(s) |
ming.tsao@uhn.on.ca
|
| Phone |
4169464501
|
| Organization name |
University of Toronto
|
| Department |
Ontario Cancer Institute
|
| Lab |
Applied Molecular Oncology
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE50081 |
Validation of a histology-independent prognostic gene signature for early stage, non-small cell lung cancer including stage IA patients |
|
