|
| Status |
Public on Jun 30, 2014 |
| Title |
Normal Volunteer, Replicate 21 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar macrophage isolated from Normal Volunteer
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Normal
tissue: Alveolar Macrophage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner.
|
| Data processing |
The data were analyzed with Affymetrix apt version 1.12, GeneSpring 11.5.1 . Apt was used to determine present/absent calls and GeneSpring RMA normalization was applied.
|
| |
|
| Submission date |
Jul 22, 2013 |
| Last update date |
Jun 30, 2014 |
| Contact name |
Eric Billings |
| Organization name |
NHLBI
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20874 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE49072 |
Alveolar Macrophage Gene Expression in Human Pulmonary Fibrosis |
|
