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Sample GSM1160138 Query DataSets for GSM1160138
Status Public on Jul 29, 2013
Title human morula 1
Sample type SRA
Source name morula
Organism Homo sapiens
Characteristics cell type: morula
ethnicity: Han Chinese
supplementary file: Human_expression_mat.txt
Treatment protocol Single cells were washed twice with 1x PBS containing 0.1% BSA before placing in lysis buffer.
Growth protocol Single blastomeres were isolated by removing the zona pellucida using acidic tyrode solution (Sigma, cat. no. T1788), then separated by gentle mouth pipetting in a calcium-free medium.
Extracted molecule total RNA
Extraction protocol RNA was isolated from single cells or single morula embryos and amplified as previously described (Tang et al., 2010, Nat Protoc)
Library construction was performed following Illumina manufacturer suggestions.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing Raw reads from the Illumina HiSeq2000 were mapped to the hg19 genome using default parameters in BWA
Mapped transcripts reads were transformed using the reads per kilobase per million (RPKM) metric
Genes with low expression in all stages (average RPKM<0.5) were filtered out, followed by quantile normalization.
Genome_build: hg19
Supplementary_files_format_and_content: human_expression_mat.txt: matrix listing normalized RPKM
Submission date Jun 11, 2013
Last update date May 15, 2019
Contact name Kevin Huang
Phone 310-267-0438
Organization name UCLA
Department Human Genetics
Lab Guoping Fan
Street address Gonda BLDG Rm. 6554, P.O.Box 957088
City Los Angeles
State/province CA
ZIP/Postal code 90095-7088
Country USA
Platform ID GPL11154
Series (1)
GSE44183 Genetic Programs in Human and Mouse Early Embryos Revealed by Single-Cell RNA-Sequencing
BioSample SAMN02195167
SRA SRX300899

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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