|
| Status |
Public on Nov 10, 2012 |
| Title |
RL |
| Sample type |
RNA |
| |
|
| Source name |
Diffuse large B-cell lymphoma cell line RL
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RL
cell type: diffuse large B-cell lymphoma (DLBCL) cells
tumor subtype: germinal center B-cell like
|
| Treatment protocol |
No treatment was performed.
|
| Growth protocol |
Cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum, 100 units/mL of penicillin and 100 mg/mL of streptomycin at 37ºC and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
The cDNA was prepared from 2 microg total RNA using the One-Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthesizing the cRNA using the IVT labeling kit (Affymetrix). The synthesized cRNA was purified with the GeneChip Sample Cleanup Module (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133 Plus 2.0 chip according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
|
| Description |
Gene expression data from a DLBCL cell line.
|
| Data processing |
Data were normalized with Bioconductor software using the RMA algorithm.
|
| |
|
| Submission date |
Nov 09, 2012 |
| Last update date |
Nov 10, 2012 |
| Contact name |
Raquel Malumbres |
| E-mail(s) |
rmalumbres@yahoo.com
|
| Phone |
+34 948194700
|
| Organization name |
University of Navarra/ Clínica Universidad de Navarra
|
| Department |
Hemato-Oncology
|
| Lab |
Multiple Myeloma
|
| Street address |
Avenida Pio XII 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE42203 |
Global expression analysis of DLBCL and SMZL cell lines |
| GSE42204 |
LITAF, a BCL6 target gene, regulates autophagy in mature B-cell lymphomas |
|
