|
| Status |
Public on Jan 28, 2013 |
| Title |
PsOEt-22-Week 12 |
| Sample type |
RNA |
| |
|
| Source name |
Lesional skin biopsy from psoriasis patient, Week12_etanercept
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Lesional skin
time: Week12_etanercept
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction.
|
| Label |
biotin
|
| Label protocol |
Total RNA was reverse-transcribed and amplified using the NuGen Ovation™ RNA amplification system V2 with Ovation™WB reagent. Amplified RNA was fragmented and labeled using the NuGen FL-Ovation™ cDNA Biotin Module V2. Amplification and labeling were performed on a Beckman-Coulter ArrayPlex.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix protocol.
|
| Scan protocol |
Procedure used standard Affymetrix protocol.
|
| Description |
M_LS_Wk12 in GSE11903 (GSM300825)
|
| Data processing |
Scans were imported into Rosetta Resolver and pre-processed using the 'Resolver Affymetrix Rosetta Intensity Profile Builder (saves reporters)' pipeline
|
| |
|
| Submission date |
Oct 17, 2012 |
| Last update date |
Jan 29, 2013 |
| Contact name |
Jeannette Bigler |
| E-mail(s) |
jeannette.bigler@bjgroupllc.com
|
| Organization name |
BJ Group, LLC
|
| Street address |
3200 NE 92nd Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE41663 |
Re-analysis by microarray using cDNA target of samples from psoriasis patients enrolled in an etanercept trial |
| GSE41664 |
Comparison of Gene Expression in Psoriatic Skin from Different Sources |
|
