|
| Status |
Public on Sep 10, 2014 |
| Title |
Eµ-myc Lymphoma Train 27 |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoma
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Eµ-myc
tissue type: lymphoma tumor
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lymphoma tissue was homogenized with a mortar and pestle and with sonication. RNA was extracted using the Rneasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Target preparation from total RNA was performed using T7 primers in the cDNA synthesis step, followed by in vitro transcription to produce biotin-labeled cRNA.
|
| |
|
| Hybridization protocol |
The cRNA was fragmented and mixed into a hybridization cocktail before application to the array, which was then hybridized for 16 hours at 45C with rotation at 60 rpm.
|
| Scan protocol |
Immediately following hybridization, the arrays underwent an automated washing and staining protocol on the GeneChip FS450 fluidics station and were then scanned on the GeneChip Scanner 3000 7G Plus.
|
| Data processing |
The data were analyzed with RMA normalization using the standard settings in the affy package from Bioconductor using R.
|
| |
|
| Submission date |
Sep 10, 2012 |
| Last update date |
Sep 10, 2014 |
| Contact name |
Daphne R Friedman |
| E-mail(s) |
daphne.friedman@duke.edu
|
| Organization name |
Duke University Medical Center
|
| Street address |
101 Science Drive, Room 2121, CIEMAS Bldg
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE40756 |
Transgenic Eµ-myc mouse lymphoma expression data for training dataset |
| GSE40760 |
The Eµ-myc Mouse Model Represents Heterogeneity Across Human Aggressive B-cell Lymphomas |
|
