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Series GSE99350 Query DataSets for GSE99350
Status Public on Feb 21, 2018
Title A Signaling and Transcription Architecture for the Specification of Human Germ Cell Lineage
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The mechanism for human germ-cell specification, which sets out a complex program generating spermatozoa or oocytes, remains largely unknown. We established a system for inducing human primordial germ cell-like cells (hPGCLCs) from induced pluripotent stem cells (hiPSCs) via incipient mesoderm-like cells (iMeLCs). Here, we show that EOMESODERMIN (EOMES) elevates in iMeLCs through WNT signaling and is essential for activating SOX17, a key driver for hPGC(LC) specification, with the duration/dosage of the WNT signaling/EOMES expression dictating the germ-cell competence. Upon hPGCLC induction, BMP signaling activates TFAP2C independently from SOX17, and SOX17 and TFAP2C instate the hPGCLC program, including BLIMP1 expression, in an interdependent fashion. The hPGC(LC) specification program is highly consistent with the monkey program, but is divergent from the mouse one regarding key transcription factors and their hierarchy of actions. These findings delineate evolutionary divergence of mammalian germ-cell specification, providing a foundation for further human germ-cell development in vitro.
Overall design RNAseq analysis of hiPSCs, iMeLCs and hPGCLCs in multiple knockout/rescued lines
Contributor(s) Kojima Y, Sasaki K, Yokobayashi S, Nakamura T, Yabuta Y, Nakaki F, Nagaoka S, Woltjen K, Hotta A, Yamamoto T, Saitou M
Citation(s) 28985527
Submission date May 26, 2017
Last update date May 15, 2019
Contact name Yukihiro Yabuta
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (230)
GSM2642567 Ctrl_SOX17/EOMES, iPSC, MS081Y77
GSM2642568 Ctrl_SOX17/EOMES, iPSC, MS082Y08
GSM2642569 Ctrl_SOX17/EOMES, iPSC, MS082Y17
BioProject PRJNA388179
SRA SRP108121

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Supplementary file Size Download File type/resource
GSE99350_expression_RNAseq_Kojima_hg19_RPM_v1.txt.gz 12.4 Mb (ftp)(http) TXT
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