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| Status |
Public on Jun 13, 2017 |
| Title |
Expression data of growing and senescent human fibroblasts (BJ cells) after p21 knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cellular senescence is a permanent state of cell cycle arrest that protects the organism from tumorigenesis and regulates tissue integrity upon damage and during tissue remodeling. However, accumulation of senescent cells in tissues during aging contributes to age-related pathologies.
A deeper understanding of the mechanisms regulating the viability of senescent cells is therefore required. Here we show that the CDK inhibitor p21 (CDKN1A) maintains the viability of DNA damage-induced senescent cells.
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| Overall design |
To identify the molecular mechanism controlling senescence cell viability, we studied the expression patterns of damage induced senescent (DIS) and control cells with and without p21 knockdown.
Growing and DIS BJ cells were transfected with siRNAs against p21 or control siRNAs. After 3 days, total RNA was extracted and gene expression was determined using Affymetrix microarrays.
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| Contributor(s) |
Yosef R |
| Citation(s) |
28607003 |
| Submission date |
Apr 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Valery Krizhanovsky |
| Organization name |
Weizmann Institute of Science
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| Department |
Molecular Cell Biology
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| Street address |
Herzel 234
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| City |
Rehovot |
| ZIP/Postal code |
761000 |
| Country |
Israel |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (12)
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| GSM2589746 |
Growing BJ cells transfected with siCtrl, biological repeat 1 |
| GSM2589747 |
Growing Bj cells transfected with siCtrl, biological repeat 2 |
| GSM2589748 |
Growing Bj cells transfected with sip21, biological repeat 1 |
| GSM2589749 |
Growing Bj cells transfected with sip21, biological repeat 2 |
| GSM2589750 |
Growing BJ cells transfected with siCtrl, biological repeat 3 |
| GSM2589751 |
Growing Bj cells transfected with sip21, biological repeat 3 |
| GSM2589752 |
Senescent BJ cells transfected with siCtrl, biological repeat 1 |
| GSM2589753 |
Senescent BJ cells transfected with sip21, biological repeat 1 |
| GSM2589754 |
Senescent BJ cells transfected with sip21, biological repeat 2 |
| GSM2589755 |
Senescent BJ cells transfected with siCtrl, biological repeat 2 |
| GSM2589756 |
Senescent BJ cells transfected with siCtrl, biological repeat 3 |
| GSM2589757 |
Senescent BJ cells transfected with sip21, biological repeat 3 |
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| Relations |
| BioProject |
PRJNA384368 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98240_RAW.tar |
22.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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