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| Status |
Public on Sep 12, 2017 |
| Title |
Cell-cycle-resolved analysis of RNA expression in neuroblastoma cells upon MYCN knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous studies of c-MYC or MYCN effects on the cellular transcriptome have been performed with unsynchronized tumor cell populations. We reasoned that these results might have been influenced by different cell-cycle profiles of cells with high or low MYC levels as the expression of a large part of the genome varies with cell-cycle phase. To correct for this potentially confounding effect, we performed RNA sequencing in cell-cycle-synchronized neuroblastoma cells and then compared the transcriptomes of cells with high and low MYCN in equivalent cell-cycle phases.
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| Overall design |
IMR5/75 neuroblastoma cells with tetracycline-inducible MYCN shRNA were arrested at G1:S transition by thymidine treatment for 18 h. After washout, transcriptome was measured at 11 time points over 22h (2 biological replicates).
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| Contributor(s) |
Bell E, Friedrich M |
| Citation(s) |
28843484 |
| Submission date |
Apr 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chunxuan Shao |
| E-mail(s) |
c.shao@Dkfz-Heidelberg.de
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| Organization name |
German Cancer Research Center
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| Street address |
Im Neuenheimer Feld 280
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (44)
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| Relations |
| BioProject |
PRJNA383104 |
| SRA |
SRP104120 |
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