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Series GSE96806 Query DataSets for GSE96806
Status Public on Apr 28, 2017
Title Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transcription factors LEAFY (LFY) and APETALA1 (AP1), together with the AP1 paralog CAULIFLOWER (CAL), control the onset of flower development in a partially redundant manner. This redundancy is thought to be mediated, at least in part, through the regulation of a shared set of target genes. However, whether these genes are independently or cooperatively regulated by LFY and AP1/CAL, is currently unknown. To better understand the regulatory relationship between LFY and AP1/CAL during floral initiation, we monitored the activity of LFY in the absence of AP1/CAL function. We found that the regulation of several known LFY target genes is unaffected by AP1/CAL perturbation, while others appear to require AP1/CAL activity. Furthermore, we obtained evidence that LFY and AP1/CAL control the expression of some genes in an antagonistic manner. Notably, these include key regulators of floral initiation such TERMINAL FLOWER1 (TFL1), which had been previously reported to be directly repressed by both LFY and AP1. We show here that TFL1 expression is suppressed by AP1 but promoted by LFY. We further demonstrate that LFY has an inhibitory effect on flower formation in the absence of AP1/CAL activity. We propose that LFY and AP1/CAL may act as part of an incoherent feed-forward loop to control the establishment of a stable developmental program for the formation of flowers.
Overall design Two ChIP-Seq datasets performed in Arabidopsis lines expressing a LFY-GR construct. The first ChIP uses a LFY specific polyclonal AB generated by the authors. The second is an input control with no exposure to the LFY specific AB.
Contributor(s) Goslin K, Zheng B, Serrano-MIslata A, Rae L, Ryan PT, Kwasniewska K, Thomson B, Ó'Maoiléidigh DS, Madueño F, Wellmer F, Graciet E
Citation(s) 28385730
Submission date Mar 20, 2017
Last update date May 15, 2019
Contact name Pat Ryan
Organization name TCD
Street address College Green
City Dublin
ZIP/Postal code na
Country Ireland
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (2)
GSM2544348 LFYGR
BioProject PRJNA379771
SRA SRP102171

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Supplementary file Size Download File type/resource
GSE96806_annotateLFYGR2.csv.gz 39.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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