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Series GSE9674 Query DataSets for GSE9674
Status Public on Feb 29, 2008
Title Expression data from Arabidopsis plants misexpressing AtMYB30 after Xanthomonas inoculation at early timepoints
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Plant immune responses to pathogen attack involve various defense mechanisms and among them, the Hypersensitive Response (HR), a form of programmed cell death occurring at invasion sites. AtMYB30, a transcription factor acts as a positive regulator of a cell death pathway conditioning the HR.
We show by microarray analyses of Arabidopsis plants misexpressing AtMYB30 that the genes encoding the four enzymes forming the acyl-coA elongase complex, responsible for very long chain fatty acids (VLCFA) biosynthesis, are putative targets.
Keywords: Time course after inoculation with a Xanthomonas strain, Xcc147, in Arabidopsis wild-type plants and transgenic plants (AtMYB30 overexpressor (ox) and antisense (as) lines
Overall design 18 samples are analyzed : Leaves of 4 week-old plants syringe-infiltrated with the Xcc147 strain (or not) were harvested. In experiment 1, we selected 4 timepoints : T0 (not inoculated), 90-105 min, 2-4 h and 6 h post inoculation in the wild-type line, and in a transgenic line overexpressing AtMYB30. Experiment 2 is a biological replicate of experiment 1 (except for 6 hpi). In experiment 3, we focused on the early timepoint 90-105 min to compare the wild-type line and an AtMYB30 antisense line. Experiment 4 is a biological replicate of experiment 3. Plant material was pooled when necessary, and frozen before RNA extraction and hybridization on Affymetrix ATH1 microarrays. As a screen for candidate target genes, we looked for genes, i) up-regulated in the wild-type line after inoculation with Xcc147 as compared to time T0 (not inoculated) , ii) over-expressed in the AtMYB30 overexpressor line compared to the wild-type, and iii) down-regulated in the AtMYB30 antisense line compared to the wild-type.
Contributor(s) Raffaele S, Vailleau F, Léger A, Joubes J, Miersch O, Huard C, Blée E, Mongrand S, Domergue F, Roby D
Citation(s) 18326828
Submission date Nov 23, 2007
Last update date Aug 28, 2018
Contact name Dominique ROBY
Phone 33 05 61 28 55 11
Fax 33 05 61 28 50 61
Organization name CNRS/INRA
Lab Laboratoire des Interactions Plantes - Microorganismes
Street address Chemin de Borde Rouge
City Castanet-Tolosan
ZIP/Postal code F-31326
Country France
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (18)
GSM244442 Arabidopsis wild-type_T0 (not inoculated)_biological rep1_exp1
GSM244443 Arabidopsis wild-type_T0 (not inoculated)_biological rep2_exp2
GSM244444 Arabidopsis wild-type_90-105 min_ Xanthomonas inoculated_biological rep1_exp1
Affiliated with GSE69995
BioProject PRJNA103561

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Supplementary file Size Download File type/resource
GSE9674_RAW.tar 58.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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