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| Status |
Public on Feb 29, 2008 |
| Title |
Expression data from Arabidopsis plants misexpressing AtMYB30 after Xanthomonas inoculation at early timepoints |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Plant immune responses to pathogen attack involve various defense mechanisms and among them, the Hypersensitive Response (HR), a form of programmed cell death occurring at invasion sites. AtMYB30, a transcription factor acts as a positive regulator of a cell death pathway conditioning the HR.
We show by microarray analyses of Arabidopsis plants misexpressing AtMYB30 that the genes encoding the four enzymes forming the acyl-coA elongase complex, responsible for very long chain fatty acids (VLCFA) biosynthesis, are putative targets.
Keywords: Time course after inoculation with a Xanthomonas strain, Xcc147, in Arabidopsis wild-type plants and transgenic plants (AtMYB30 overexpressor (ox) and antisense (as) lines
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| Overall design |
18 samples are analyzed : Leaves of 4 week-old plants syringe-infiltrated with the Xcc147 strain (or not) were harvested. In experiment 1, we selected 4 timepoints : T0 (not inoculated), 90-105 min, 2-4 h and 6 h post inoculation in the wild-type line, and in a transgenic line overexpressing AtMYB30. Experiment 2 is a biological replicate of experiment 1 (except for 6 hpi). In experiment 3, we focused on the early timepoint 90-105 min to compare the wild-type line and an AtMYB30 antisense line. Experiment 4 is a biological replicate of experiment 3. Plant material was pooled when necessary, and frozen before RNA extraction and hybridization on Affymetrix ATH1 microarrays. As a screen for candidate target genes, we looked for genes, i) up-regulated in the wild-type line after inoculation with Xcc147 as compared to time T0 (not inoculated) , ii) over-expressed in the AtMYB30 overexpressor line compared to the wild-type, and iii) down-regulated in the AtMYB30 antisense line compared to the wild-type.
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| Contributor(s) |
Raffaele S, Vailleau F, Léger A, Joubes J, Miersch O, Huard C, Blée E, Mongrand S, Domergue F, Roby D |
| Citation(s) |
18326828 |
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| Submission date |
Nov 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Dominique ROBY |
| E-mail(s) |
dominique.roby@toulouse.inra.fr
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| Phone |
33 05 61 28 55 11
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| Fax |
33 05 61 28 50 61
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| Organization name |
CNRS/INRA
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| Lab |
Laboratoire des Interactions Plantes - Microorganismes
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| Street address |
Chemin de Borde Rouge
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| City |
Castanet-Tolosan |
| ZIP/Postal code |
F-31326 |
| Country |
France |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (18)
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| GSM244442 |
Arabidopsis wild-type_T0 (not inoculated)_biological rep1_exp1 |
| GSM244443 |
Arabidopsis wild-type_T0 (not inoculated)_biological rep2_exp2 |
| GSM244444 |
Arabidopsis wild-type_90-105 min_ Xanthomonas inoculated_biological rep1_exp1 |
| GSM244445 |
Arabidopsis wild-type_90-105 min_ Xanthomonas inoculated_biological rep2_exp2 |
| GSM244446 |
Arabidopsis wild-type_2-4 h_ Xanthomonas inoculated_biological rep1_exp1 |
| GSM244447 |
Arabidopsis wild-type_2-4 h_ Xanthomonas inoculated_biological rep2_exp2 |
| GSM244448 |
Arabidopsis wild-type_6 h_ Xanthomonas inoculated_biological rep1_exp1 |
| GSM244449 |
Arabidopsis AtMYB30-ox_T0 (not inoculated)_biological rep1_exp1 |
| GSM244450 |
Arabidopsis AtMYB30-ox_T0 (not inoculated)_biological rep2_exp2 |
| GSM244451 |
Arabidopsis AtMYB30-ox_90-105 min_ Xanthomonas inoculated_biological rep1_exp1 |
| GSM244452 |
Arabidopsis AtMYB30-ox_90-105 min_ Xanthomonas inoculated_biological rep2_exp2 |
| GSM244453 |
Arabidopsis AtMYB30-ox_2-4 h_ Xanthomonas inoculated_biological rep1_exp1 |
| GSM244454 |
Arabidopsis AtMYB30-ox_2-4 h_ Xanthomonas inoculated_biological rep2_exp2 |
| GSM244455 |
Arabidopsis AtMYB30-ox_6 h_ Xanthomonas inoculated_biological rep1_exp1 |
| GSM244456 |
Arabidopsis wild-type_90-105 min_ Xanthomonas inoculated_biological rep1_exp3 |
| GSM244457 |
Arabidopsis wild-type_90-105 min_ Xanthomonas inoculated_biological rep2_exp4 |
| GSM244458 |
Arabidopsis AtMYB30-as_90-105 min_ Xanthomonas inoculated_biological rep1_exp3 |
| GSM244459 |
Arabidopsis AtMYB30-as_90-105 min_ Xanthomonas inoculated_biological rep2_exp4 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA103561 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9674_RAW.tar |
58.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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