GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE9514 Query DataSets for GSE9514
Status Public on Nov 06, 2007
Title Changes in gene expression in response to heme deficiency and hypoxia
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary We addressed changes in gene expression profile in response to
reduced oxygen tension in wild type or heme depletion caused by
deletion of heme biosynthesis gene hem1
Keywords: Response to heme depletion and hypoxia
Overall design I.Heme depletion.The hem1 delta strain was grown for 6 hrs in YPD media supplemented with 250 uM ALA. Cells were washed three times with water to remove excess ALA. Cells were then inoculated in duplicates into 15 ml cultures of
YPD media that was supplemented either with or without ALA. Cultures were induced for 16 h before 5 ml of cells were harvested and washed
three times in DEPC water. The culture density at the time of harvest
was approximately OD600=0.5.
II.Hypoxia. BY45742 strain was grown for 6 hrs in
YPD medium and then inoculated in 15 ml the same medium and grown
under normoxic or hypoxic conditions for 12 hrs. To create hypoxic
conditions, cultures were incubated in an anaerobic chamber using a
BBL-GasPak (BD Biosciences).
Contributor(s) Protchenko O, Philpott C
Citation(s) 18326586
Submission date Nov 05, 2007
Last update date Jul 01, 2016
Contact name Olga Protchenko
Organization name National Institutes of health
Department NIDDK
Street address 10 Center Dr, Bldg.10-Rm.9B-05
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (8)
GSM241146 hem1 strain grown in YPD with 250 uM ALA (08-15-06_Philpott_YG_S98_1)
GSM241147 WT strain grown in YPD under Hypoxia (08-15-06_Philpott_YG_S98_10)
GSM241148 WT strain grown in YPD under Hypoxia (08-15-06_Philpott_YG_S98_11)
BioProject PRJNA103313

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9514_RAW.tar 15.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap