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| Status |
Public on Apr 12, 2017 |
| Title |
Oncogenic PIK3CA(H1047R) and CTNNB1(stab) in intestinal organoids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Goals of the study was to compare transcripional and phenotypic response of mouse intestinal organoid cultures to the PIK3CA(H1047R) and CTNNB1(stab) oncogenes.
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| Overall design |
Two biological replicates of organoids with transgenic tdTomato-Luciferase, tdTomato-PIK3CAH1047R, tdTomato-CTNNB1stab or td-Tomato-PIK3CAH1047R-CTNNB1stab were analysed by RNA-Seq
By comparing 7-10 x 10E7 50bp paired end reads per library we identify transcriptional alterations in the intestinal epithelium following expression of each or both oncogenes,
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| |
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| Contributor(s) |
Riemer P, Morkel M |
| Citation(s) |
28442534 |
| |
| Submission date |
Jan 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Markus Morkel |
| E-mail(s) |
markus.morkel@charite.de
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Department |
Dept. of Pathology
|
| Lab |
Laboratory of Molecular Tumour Pathology
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA363058 |
| SRA |
SRP097691 |
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