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| Status |
Public on Nov 03, 2016 |
| Title |
Analysis of the role for TgAP2IV-3 in regulating gene expression in Toxoplasma development |
| Organism |
Toxoplasma gondii |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of TgAP2IV-3 overexpression (pH7.0, pH7.8) and knockout parasites(ph8.2) in the developmental CTG (Type III) genetic background, in vitro, 32-48hrs
The role for TgAP2IV-3 was evaluated in the overexpression system of ddFKBP(DD) fusion protein with a-tubulin promotor controlled by Shield 1 (100nM)
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| Overall design |
12 total samples. Each condition presented as 2 biological replicates
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| Contributor(s) |
Hong D, Radke JB, White MW |
| Citation(s) |
28251183 |
| Submission date |
Nov 02, 2016 |
| Last update date |
Mar 07, 2017 |
| Contact name |
Dong-Pyo Hong |
| E-mail(s) |
dhong@usf.edu
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| Phone |
7132059069
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| Organization name |
University of south florida
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| Street address |
3720 Spectrum Blvd, Suite304
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| City |
Tampa |
| State/province |
Florida |
| ZIP/Postal code |
33612 |
| Country |
USA |
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| Platforms (1) |
| GPL7186 |
[Tgondiia520372] Affymetrix Toxoplasma gondii Custom GeneChip (CDF: ToxoDB version 4) |
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| Samples (12)
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| GSM2373047 |
Parent, pH 7.0, 100 nM Shield 1, 32h, replicate 1 |
| GSM2373048 |
Parent, pH 7.0, 100 nM Shield 1, 32h, replicate 2 |
| GSM2373049 |
Parent, pH 7.8, 100 nM Shield 1, 36h, replicate 1 |
| GSM2373050 |
Parent, pH 7.8, 100 nM Shield 1, 36h, replicate 2 |
| GSM2373051 |
Parent, pH 8.2, 48h, replicate 1 |
| GSM2373052 |
Parent, pH 8.2, 48h, replicate 2 |
| GSM2373053 |
AP2IV-3 overexpression, pH7.0, 100 nM Shield 1, 32h, replicate 1 |
| GSM2373054 |
AP2IV-3 overexpression, pH7.0, 100 nM Shield 1, 32h, replicate 2 |
| GSM2373055 |
AP2IV-3 overexpression, pH7.8, 100 nM Shield 1, 36h, replicate 1 |
| GSM2373056 |
AP2IV-3 overexpression, pH7.8, 100 nM Shield 1, 36h, replicate 2 |
| GSM2373057 |
AP2IV-3 knockout, pH8.2, 48h, replicate 1 |
| GSM2373058 |
AP2IV-3 knockout, pH8.2, 48h, replicate 2 |
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| Relations |
| BioProject |
PRJNA352267 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89469_RAW.tar |
11.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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