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Series GSE89008 Query DataSets for GSE89008
Status Public on Oct 27, 2016
Title Human tracheobronchial epithelial (HTBE) cell transcriptome response to infection with H1N1, H3N2, and H5N1 influenza virus.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. mRNA-Seq analysis was used to profile the cellular transcriptome of HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
Overall design Human tracheobronchial epithelial (HTBE) cells that have been isolated from normal donor airway epithelial tissue were infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus at an MOI of 5. H3N2- and H5N1-infected samples and time-matched mock-infected samples were collected in duplicates at 3, 6, 12, and 18 hrs post infection for mRNA-Seq analysis. Sample from H1N1-infected cells and mock controls were collected at 3, 6, 12, 18, and 24 hrs.
Contributor(s) Urbanowski M, Shaw M, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A
Citation(s) 30146161
NIH grant(s)
Grant ID Grant title Affiliation Name
U19 AI106754 Multiscale analysis of influenza host-pathogen interactions: Fluomics MOUNT SINAI SCHOOL OF MEDICINE Garcia-Sastre
Submission date Oct 20, 2016
Last update date May 15, 2019
Contact name Max Chang
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (52)
GSM2357066 HTBE_H3N2_MOI0_03h_1
GSM2357067 HTBE_H3N2_MOI0_03h_2
GSM2357068 HTBE_H3N2_MOI5_03h_1
BioProject PRJNA349748
SRA SRP091886

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89008_HTBE_FPKM.csv.gz 7.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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