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| Status |
Public on Oct 27, 2016 |
| Title |
Human tracheobronchial epithelial (HTBE) cell transcriptome response to infection with H1N1, H3N2, and H5N1 influenza virus. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. mRNA-Seq analysis was used to profile the cellular transcriptome of HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
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| Overall design |
Human tracheobronchial epithelial (HTBE) cells that have been isolated from normal donor airway epithelial tissue were infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus at an MOI of 5. H3N2- and H5N1-infected samples and time-matched mock-infected samples were collected in duplicates at 3, 6, 12, and 18 hrs post infection for mRNA-Seq analysis. Sample from H1N1-infected cells and mock controls were collected at 3, 6, 12, 18, and 24 hrs.
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| Contributor(s) |
Urbanowski M, Shaw M, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A |
| Citation(s) |
30146161 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U19 AI106754 |
Multiscale analysis of influenza host-pathogen interactions: Fluomics |
MOUNT SINAI SCHOOL OF MEDICINE |
Garcia-Sastre |
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| Submission date |
Oct 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Max Chang |
| E-mail(s) |
mchang@ucsd.edu
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| Organization name |
University of California, San Diego
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (52)
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| Relations |
| BioProject |
PRJNA349748 |
| SRA |
SRP091886 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89008_HTBE_FPKM.csv.gz |
7.6 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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