|
| Status |
Public on Feb 22, 2019 |
| Title |
Gene expression profiles of TN breast tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Engaging the immune system promises to be key for optimal cancer therapy, especially in hard-to-treat disease such as triple-negative breast cancer (TNBC). However, the immune microenvironment remains poorly understood. Using gene expression based on laser capture microdissection, we identify three distinct tumor microenvironments associated with CD8+ T cell localization patterns and outcome in TNBC. An immunoreactive microenvironment is defined by granzyme B-positive CD8+ T cell infiltration, a type I interferon signature, tumor expression of PD-L1 and good outcome. In contrast, tumors with an immune-cold microenvironment display restriction of CD8+ T cells to tumor margins, elevated tumor expression of the immunosuppressive marker B7-H4, a signature of desmoplastic stroma and poor outcome. A third distinct immunomodulatory microenvironment associated with poor outcome is enriched for IL-17-producing cells and neutrophils, as well as stroma-restricted localisation of CD8+ T cells. These distinct immune microenvironments have implications for TNBC patient stratification for current and future therapies.
|
| |
|
| Overall design |
37 patient samples; each subjected to gene expression profiling using Affymetrix Human Gene 1.0 ST Array
|
| |
|
| Contributor(s) |
Park M |
| Citation(s) |
30753167 |
| |
| Submission date |
Oct 17, 2016 |
| Last update date |
Feb 22, 2019 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
benjamin.haibe.kains@utoronto.ca
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL20171 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [hugene10st_Hs_ENTREZG_19.0.0] |
|
| Samples (37)
|
|
| Relations |
| BioProject |
PRJNA348766 |
Less...
More...