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Series GSE85018 Query DataSets for GSE85018
Status Public on Jun 20, 2017
Title Germinal-center development of memory B cells driven by IL-9 from follicular helper T cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A subset of GC B cells that have stopped cycling, upregulated CD38 and downregulated BCL-6 is functionally verified as GC-derived memory B cell precursors (GC-MPs). RNA-seq analyses of the transcriptome were used to probe the developmental trajectory of these cells and their responses to IL-9, a cytokine that is found to drive the memory development from the GC.
Overall design Differential gene expression analyses between GC-MP cells and regular GC B cells in G1 phase (GC-MPP cells); Gene expression profiling of different GC subsets in comparison to memory B cells and plasma cells; acute effects of in vivo IL-9 or anti-IL-9 treatment on GC-MP or GC-MPP cells.
Contributor(s) Qi H, Wang Y, Shi J, Yan J, Xiao Z, Hou X, Lu P, Hou S, Mao T, Ma Y, Zhang L, Yang X
Citation(s) 28650481
Submission date Jul 31, 2016
Last update date May 15, 2019
Contact name Hai Qi
Organization name Tsinghua University
Department School of Medicine
Lab Laboratory of Dynamic Immunobiology
Street address Tsinghuayuan Street
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (66)
GSM2256289 mKO2hi-1-1
GSM2256290 mKO2hi-1-2
GSM2256291 mKO2hi-1-3
BioProject PRJNA335909
SRA SRP080709

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85018_mKO2hi_mKO2lo_IL9_aIL9_counts.txt.gz 694.6 Kb (ftp)(http) TXT
GSE85018_mKO2hi_mKO2lo_IL9_aIL9_fpkm.txt.gz 1.7 Mb (ftp)(http) TXT
GSE85018_mKO2hi_mKO2lo_memory_PC_counts.txt.gz 619.6 Kb (ftp)(http) TXT
GSE85018_mKO2hi_mKO2lo_memory_PC_fpkm.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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