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| Status |
Public on Jun 20, 2017 |
| Title |
Germinal-center development of memory B cells driven by IL-9 from follicular helper T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A subset of GC B cells that have stopped cycling, upregulated CD38 and downregulated BCL-6 is functionally verified as GC-derived memory B cell precursors (GC-MPs). RNA-seq analyses of the transcriptome were used to probe the developmental trajectory of these cells and their responses to IL-9, a cytokine that is found to drive the memory development from the GC.
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| Overall design |
Differential gene expression analyses between GC-MP cells and regular GC B cells in G1 phase (GC-MPP cells); Gene expression profiling of different GC subsets in comparison to memory B cells and plasma cells; acute effects of in vivo IL-9 or anti-IL-9 treatment on GC-MP or GC-MPP cells.
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| Contributor(s) |
Qi H, Wang Y, Shi J, Yan J, Xiao Z, Hou X, Lu P, Hou S, Mao T, Ma Y, Zhang L, Yang X |
| Citation(s) |
28650481 |
| Submission date |
Jul 31, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hai Qi |
| E-mail(s) |
qihai@mail.tsinghua.edu.cn
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| Organization name |
Tsinghua University
|
| Department |
School of Medicine
|
| Lab |
Laboratory of Dynamic Immunobiology
|
| Street address |
Tsinghuayuan Street
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (66)
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| Relations |
| BioProject |
PRJNA335909 |
| SRA |
SRP080709 |
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