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Series GSE82322 Query DataSets for GSE82322
Status Public on Jun 08, 2016
Title elli-1 gene expression array
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Germ cells contain non-membrane bound cytoplasmic organelles that help maintain germline integrity. In C. elegans they are called P granules; without them, the germline undergoes partial masculinization or aberrant differentiation. One key P-granule component is the Argonaute CSR-1, a small-RNA binding protein that antagonizes accumulation of sperm-specific transcripts in developing oocytes. Loss of CSR-1 or components of its pathway results in a very specific, enlarged P-granule phenotype. In a forward screen to identify mutants with abnormal P granules, ten alleles were recovered with csr-1 P-granule phenotypes, eight of which contain mutations in known CSR-1 pathway components (csr-1, ego-1, ekl-1, and drh-3). The remaining two alleles are in a novel gene now called elli-1 (enlarged germline granules). ELLI-1 is first expressed in primordial germ cells during mid-embryogenesis and continues to be expressed in the adult germline. While ELLI-1 forms cytoplasmic aggregates, they occasionally dock, but do not co-localize with P granules. Instead, the majority of ELLI-1 aggregates accumulate in the shared germline cytoplasm. In elli-1 mutants, several genes that promote RNAi and P-granule accumulation are upregulated, and embryonic lethality, sterility, and RNAi resistance in a hypomorphic drh-3 allele is enhanced. Like mutations in other CSR-1 pathway components, elli-1 is an enhancer of glp-1 sterility, suggesting that ELLI-1 functions with the CSR-1 pathway to modulate RNAi activity, P-granule accumulation, and post-transcriptional expression in the germline.
Overall design Four RNA samples from strain DUP9 elli-1(sam3) were compared to four RNA samples from strain DUP17 (control).
Contributor(s) Andralojc KM, Updike DL
Citation(s) 28182654
Submission date Jun 07, 2016
Last update date Jul 10, 2019
Contact name Dustin Lynn Updike
Phone 2082889880
Organization name Mount Desert Island Biological Laboratory
Lab Updike Lab
Street address 159 Old Bar Harbor Rd.
City Salisbury Cove
State/province ME
ZIP/Postal code 04672
Country USA
Platforms (1)
GPL21109 Agilent-068300 ArrayXS_Celegans_OakLabs_06-2014_V2 (SystematicName version)
Samples (8)
GSM2190182 C. elegans_elli-1_rep1
GSM2190183 C. elegans_elli-1_rep2
GSM2190184 C. elegans_elli-1_rep3
BioProject PRJNA324647

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Supplementary file Size Download File type/resource
GSE82322_RAW.tar 89.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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