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Status |
Public on Jul 01, 2016 |
Title |
Motor cortex after C3 lesion |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Gene expression analysis of motor cortex after spinal C3 lesion
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Overall design |
Dorsal column wire knife lesions: Adult female Fischer 344 rats weighing 150-200 gm were used. Animals underwent a laminectomy at spinal level C3. Dorsal funiculus lesions were made in the middle of C3 using a Kopf microwire device (Kopf Instruments, Tujunga, CA). After fixation in a spinal stereotaxic unit, a small dural incision was made. The wire knife was lowered into the spinal cord to a depth of 1.1 mm ventral to the dorsal cord surface and 1.1 mm to the left of the midline. The tip of the wireknife was extruded, forming a 2.25 mm-wide arc that was raised to the dorsal surface of the cord. To ensure complete axotomy of the dorsal funiculus, spinal tissue was compressed against the microwire knife surface using a microaspiration pipette until all visible white matter was transected. Cortical microdissection: The forelimb and hindlimb motor cortex were microdisected from rat cortices: Rostral to Bregma: an area from 2.0 to 4.5 mm mediolateral and from 0 to 2 mm anterior-posterior Caudal to Bregma: an area from 2.0 to 3.5 mediolateral and from 0 to 3 mm caudal to Bregma. Only the inferior half of the cortex containing layer V corticospinal motor neurons was sampled in each region.
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Contributor(s) |
Tuszynski M, Blesch A |
Citation(s) |
26898779 |
Submission date |
Jan 08, 2016 |
Last update date |
Jul 31, 2017 |
Contact name |
Giovanni Coppola |
E-mail(s) |
gcoppola@ucla.edu
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Phone |
310-794-4172
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Organization name |
UCLA
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Department |
Psychiatry and Neurology
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Lab |
Neurogenetics
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Street address |
1524 Gonda, 695 Charles Young Drive South
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City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
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Platforms (1) |
GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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Samples (19)
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Relations |
BioProject |
PRJNA308308 |