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Series GSE74912 Query DataSets for GSE74912
Status Public on Jun 01, 2016
Title ATAC-seq data
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A single hematopoietic stem cell can give rise to all blood cells with remarkable fidelity. Here, we define the chromatin accessibility and transcriptional landscape controlling this process in thirteen primary cell types that traverse the hematopoietic hierarchy. Exploiting the finding that enhancer landscapes better reflect cell identity than mRNA levels, we enable "enhancer cytometry" for accurate enumeration of pure cell types from complex populations. We further reveal the lineage ontogeny of genetic elements linked to diverse human diseases. In acute myeloid leukemia, chromatin accessibility reveals distinctive regulatory evolution in pre-leukemic HSCs (pHSCs), leukemia stem cells, and leukemic blasts. These leukemic cells demonstrate unique lineage infidelity, confirmed by single cell regulomes. We further show that pHSCs have a competitive advantage that is conferred by reduced chromatin accessibility at HOXA9 targets and is associated with adverse patient outcomes. Thus, regulome dynamics can provide diverse insights into human hematopoietic development and disease.
Overall design ATAC-seq profiles of hematopoietic and leukemic cell types, across 13 normal hematopoietic cell types and 3 acute myeloid leukemia cell types. The complete data set contains a total of 132 samples.
Contributor(s) Buenrostro J
Citation(s) 27526324, 33686069
Submission date Nov 11, 2015
Last update date Apr 07, 2021
Contact name Jason Daniel Buenrostro
Organization name Broad Institute
Street address 415 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (130)
GSM1937376 4983-1A
GSM1937377 4983-2A
GSM1937378 4983-3A
This SubSeries is part of SuperSeries:
GSE75384 Lineage-specific and single cell chromatin accessibility charts human hematopoiesis and leukemia evolution
BioProject PRJNA301969
SRA SRP066100

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Supplementary file Size Download File type/resource
GSE74912_ATACseq_All_Counts.txt.gz 63.3 Mb (ftp)(http) TXT
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