|
| Status |
Public on Jun 02, 2017 |
| Title |
C/EBPepsilon ChIP-sequencing of C57BL/6 mice bone marrow cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To characterize the genome wide C/EBPepsilon binding sites, ChIP-sequencing with C57BL/6 mice bone marrow cells were performed. To prevent artifact from PCR amplifiation, sequencing or antibody specificity, two controls of input DNA and ChIP-sequencing of C/EBPepsilon knock-out bone marrow cells were used. Two data sets derived from two anti-C/EBPepsilon antibodies, N-terminal (GTX109155) and C-terminal (SC-158) antibody against wild type and C/EBPepsilon knock-out bone marrow cells were generated.
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| |
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| Overall design |
Two C terminal and N terminal antibodies against C/EBPepsilon on C57BL/6 wild type and C/EBPepsilon knock-out mice bone marrow cells
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| |
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| Contributor(s) |
Suh HC, Benoukraf T, Yin T, Cao Q, Said J, Lee S, Lim R, Yang H, Shyamsunder P, Madan V, Koeffler HP |
| Citation(s) |
28440307 |
| Submission date |
Oct 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Touati Benoukraf |
| E-mail(s) |
tbenoukraf@mun.ca
|
| Phone |
+1 (709) 864-6671
|
| Organization name |
Memorial University of Newfoundland
|
| Department |
Faculty of Medicine, Discipline of Genetics
|
| Street address |
Craig L. Dobbin Genetics Research Centre,
|
| City |
St. John's |
| State/province |
NL |
| ZIP/Postal code |
A1B 3V6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
| GSM1904411 |
CEBPe Chipseq (GTX)-Whole bone marrow-B6 mouse |
| GSM1904412 |
CEBPe Chipseq (GTX)-Whole bone marrow-CEBPe KO mouse |
| GSM1904413 |
Input-Whole bone marrow-B6 mouse |
| GSM1904414 |
Input-Whole bone marrow-CEBPe KO mouse |
| GSM1904415 |
CEBPe Chipseq (SC158)-Whole bone marrow-B6 mouse |
| GSM1904416 |
CEBPe Chipseq (SC158)-Whole bone marrow-CEBPe KO mouse |
|
| This SubSeries is part of SuperSeries: |
| GSE73860 |
Landscape of CEBPe target genes in mouse bone marrow |
|
| Relations |
| BioProject |
PRJNA298234 |
| SRA |
SRP064619 |
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