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Series GSE7379 Query DataSets for GSE7379
Status Public on Aug 15, 2007
Title Fis KO strain
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary Fis is a nucleoid-associated protein in E. coli that is abundant during early logarithmic growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E. coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild type strains during early log, mid log, late log, and stationary growth phases.
We used microarrays to detail the global impact of Fis on gene expression in Escherichia coli
Keywords: time course
 
Overall design E.coli cells were were grown and samples were taken at different times during growth: 90 min (early logarithmic phase), 150 min (mid-logarithmic phase), 240 min (late logarithmic phase), and 360 min (early stationary phase) for RNA extraction and hybridization on Affymetrix microarrays. This was done in triplicate and the raw data was analyzed using Microarray Analysis Suite version 5.0 (Affymetrix).
 
Contributor(s) Osuna R, Bradley MD, Beach MB, Pratt TS
Citation(s) 17768236
Submission date Mar 27, 2007
Last update date Jul 26, 2013
Contact name Robert Osuna
E-mail(s) osuna@albany.edu
Phone 518-591-8827
Fax 518-442-4767
Organization name University at Albany
Department Biology
Lab Dr. Osuna
Street address 1400 Washington Avenue
City Albany
State/province NY
ZIP/Postal code 12222
Country USA
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (12)
GSM177549 fis 90 minutes growth rep1
GSM177550 fis 150 minutes growth rep1
GSM177551 fis 240 minutes growth rep1
This SubSeries is part of SuperSeries:
GSE7398 Expression data from E. coli covering four phases of growth in Fis KO and WT (Fis+) strains
Relations
BioProject PRJNA104083

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7379_RAW.tar 25.6 Mb (http)(custom) TAR (of CEL)
GSE7379_README.TXT 271 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data provided as supplementary file

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