|
| Status |
Public on Nov 21, 2016 |
| Title |
The SWI/SNF chromatin remodeling complex is required for lineage specific enhancer activity [MEF_RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Genes encoding subunits of SWI/SNF (BAF) chromatin remodeling complexes are collectively altered in over 20% of all human malignancies, but the mechanisms by which these complexes alter chromatin to modulate transcription and control cell fate are poorly understood. Utilizing both loss-of-function and gain-of-function approaches, here we show that SWI/SNF complexes are preferentially targeted to distal enhancers and interact with p300 to regulate transcription via modulation of histone H3 lysine 27 acetylation. We identify a greater requirement for SWI/SNF at typical enhancers than at most super-enhancers and at enhancers in untranscribed regions than in transcribed regions. Our data further demonstrate that SWI/SNF-dependent distal enhancers are essential for controlling expression of genes linked to developmental processes. Our findings thus establish SWI/SNF complexes as regulators of the enhancer landscape and provide insight into the roles of SWI/SNF in cellular fate control.
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| |
|
| Overall design |
RNA-seq in Mouse Embryonic Fibroblasts in WT condition and for knockouts of different SWI/SNF complex subunits.
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| |
|
| Contributor(s) |
Kim KH, Alver BH, Lu P, Park PJ, Roberts CW |
| Citation(s) |
28262751 |
| Submission date |
Jul 29, 2015 |
| Last update date |
Sep 27, 2019 |
| Contact name |
Peter J Park |
| E-mail(s) |
peter_park@harvard.edu
|
| Phone |
617-432-7373
|
| Organization name |
Harvard Medical School
|
| Department |
Center for Biomedical Informatics
|
| Street address |
10 Shattuck St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE71509 |
The SWI/SNF chromatin remodeling complex is required for lineage specific enhancer activity |
|
| Relations |
| BioProject |
PRJNA291413 |
| SRA |
SRP061774 |
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