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Series GSE71478 Query DataSets for GSE71478
Status Public on Feb 26, 2016
Title Expression data from E12.5 male fatal PGCs, PGCLC in vitro, round spermatids and spermatids like cells produced in vitro
Organism Mus musculus
Experiment type Expression profiling by array
Summary Generation of haploid gametes in vitro can potentially address gamete failure-based infertility.This study reports complete in vitro meiosis from murine ESC-derived PGCLCs resulting in the formation of male spermatid-like cells (SLCs) capable of producing viable fertile offspring via intracytoplasmic sperm injection (ICSI).Our findings provide the basis for generation of haploid spermatids in vitro in human, the generation of transgenic animals, and the use of this system to investigate mechanisms of meiosis.
Overall design We used microarrays to compare gene expression profiles of in vivo and in vitro derived PGC cells and round spermatids.
We collected E12.5 male fatal PGCs, PGCLC in vitro, round spermatids and spermatids like cells produced in vitro, each sample has 3 replications.
Contributor(s) Zhou Q, Sha J, Zhao X
Citation(s) 26923202
Submission date Jul 29, 2015
Last update date Feb 11, 2019
Contact name Quan Zhou
Organization name Nanjing Medical University
Street address NO. 140, Hanzhong Road
City Nanjing
State/province Jiangsu
ZIP/Postal code 210000
Country China
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM1835316 E12.5 male PGCs, biological rep1
GSM1835317 E12.5 male PGCs, biological rep2
GSM1835318 E12.5 male PGCs, biological rep3
BioProject PRJNA291354

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71478_RAW.tar 47.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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