GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE65523 Query DataSets for GSE65523
Status Public on Feb 02, 2018
Title RNA sequencing (RNA-SEQ) of PSPC1 knockdown by shRNA in A549 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We show that PSPC1 is required for cancer cell motilities and stemness properties in vitro and lung metastasis in vivo. We used high throughput sequencing to analyze the PSPC1 regulated gene expression. Loss of PSPC1 results in significant gene expression level changes in thousands of individual transcripts in key regulators of the EMT, CSCs and TGFb signaling.
Overall design Methods: Gene expression profiles of A549 shPSPC1 knockdown cells were generated by Illumna RNA sequencing and compared to profiles derived from control cells (shLacZ knockdown).
Contributor(s) Yeh HW, Jou YS
Citation(s) 29593326
Submission date Feb 02, 2015
Last update date May 15, 2019
Contact name HSI-Wen Yeh
Phone 886-2-26523525
Organization name Academia Sinica
Department Institute of biomedical science
Street address Academia Sinica, No. 128
City Taipei
State/province TAIWAN
ZIP/Postal code 115
Country Taiwan
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (2)
GSM1599488 Sample A
GSM1599489 Sample AI
BioProject PRJNA274272
SRA SRP053050

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65523_RAW.tar 16.7 Mb (http)(custom) TAR (of GTF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap