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Series GSE64245 Query DataSets for GSE64245
Status Public on Apr 08, 2016
Title A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Multicellular organisms develop new structures by initiating specific gene regulatory programs through coordinated changes at the transcriptional and chromatin levels. Master transcription factors orchestrate such global changes but the underlying molecular mechanisms remain unclear. Here we focus on LEAFY (LFY), a key regulator of flower development in angiosperms. The resolution of the crystallographic structure of its N-terminal domain shows that it forms an unanticipated Sterile Alpha Motif (SAM) domain. This domain drives LFY oligomerization, which we establish to be essential for LFY floral switch function. Combining in vitro and in vivo experiments, we demonstrate that oligomerization facilitates LFY binding to DNA regions with multiple binding sites. Moreover, genome-wide analyses reveal an additional prominent role for the oligomerization domain: it confers upon LFY the capacity to bind to closed chromatin regions. This novel property, combined with its previously demonstrated capacity to recruit chromatin remodelers, indicates that LFY controls floral fate by acting as a plant pioneer transcription factor.
Overall design A. thaliana LEAFY oligomerization ChIP-seq vs. control, 2 replicates.
Contributor(s) Sayou C, Nanao MH, Jamin M, Posé D, Thévenon E, Grégoire LS, Tichtinsky G, Denay G, Ott F, Llobet Peirats M, Schmid M, Dumas R, Parcy F
Citation(s) 27097556
Submission date Dec 16, 2014
Last update date May 15, 2019
Contact name Francois Parcy
Organization name CNRS
Lab Physiologie Cellulaire et Végétale
Street address 17, av. des Martyrs
City Grenoble
ZIP/Postal code 38054
Country France
Platforms (1)
GPL9302 Illumina Genome Analyzer II (Arabidopsis thaliana)
Samples (6)
GSM1566964 p35S:LFY, rep1
GSM1566965 p35S:LFY, rep2
GSM1566966 p35S:LFYTERE, rep1
BioProject PRJNA270526
SRA SRP051214

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Supplementary file Size Download File type/resource
GSE64245_peaks_p35SLFY.bed.gz 28.5 Kb (ftp)(http) BED
GSE64245_peaks_p35SLFYTERE.bed.gz 2.7 Kb (ftp)(http) BED
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