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| Status |
Public on Apr 16, 2015 |
| Title |
Time-series of the re-establishment of desiccation tolerance by ABA in germinated Arabidopsis thaliana seeds |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose DT while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with abscisic acid (ABA).
We used a time-series of microarrays to gain temporal resolution and identify relevant genes in the re-establishment of desiccation tolerance with ABA.
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| Overall design |
Germinated Arabidopsis seeds at the stage of radicle protrusion were incubated in ABA for 0h, 2h, 12h, 24h and 72h for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates represented by pools of seeds bulk-harvested from at least two plants were used.
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| Contributor(s) |
Costa MC, Righetti K, Ligterink W, Buitink J, Hilhorst HW |
| Citation(s) |
25809152, 26484244 |
| Submission date |
Oct 30, 2014 |
| Last update date |
Oct 21, 2015 |
| Contact name |
Maria Cecilia Costa |
| E-mail(s) |
maria.diascosta@wur.nl
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| Organization name |
Wageningen University
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| Department |
Plant Physiology
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| Lab |
Wageningen Seed Lab
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| Street address |
Droevendaalsesteeg, 1
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| City |
Wageningen |
| State/province |
The Netherlands |
| ZIP/Postal code |
6708PB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL19371 |
[AraGene-1_1-st] Arabidopsis Gene 1.1 ST Array [CDF: aragene11st_At_TAIRT.cdf version 17] |
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| Samples (15)
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| GSM1535443 |
2h incubation, biological rep1 |
| GSM1535444 |
2h incubation, biological rep2 |
| GSM1535445 |
2h incubation, biological rep3 |
| GSM1535446 |
12h incubation, biological rep1 |
| GSM1535447 |
12h incubation, biological rep2 |
| GSM1535448 |
12h incubation, biological rep3 |
| GSM1535449 |
24h incubation, biological rep1 |
| GSM1535450 |
24h incubation, biological rep2 |
| GSM1535451 |
24h incubation, biological rep3 |
| GSM1535452 |
72h incubation, biological rep1 |
| GSM1535453 |
72h incubation, biological rep2 |
| GSM1535454 |
72h incubation, biological rep3 |
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| Relations |
| BioProject |
PRJNA265873 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62876_RAW.tar |
93.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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