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Series GSE62876 Query DataSets for GSE62876
Status Public on Apr 16, 2015
Title Time-series of the re-establishment of desiccation tolerance by ABA in germinated Arabidopsis thaliana seeds
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose DT while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with abscisic acid (ABA).
We used a time-series of microarrays to gain temporal resolution and identify relevant genes in the re-establishment of desiccation tolerance with ABA.
Overall design Germinated Arabidopsis seeds at the stage of radicle protrusion were incubated in ABA for 0h, 2h, 12h, 24h and 72h for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates represented by pools of seeds bulk-harvested from at least two plants were used.
Contributor(s) Costa MC, Righetti K, Ligterink W, Buitink J, Hilhorst HW
Citation(s) 25809152, 26484244
Submission date Oct 30, 2014
Last update date Oct 21, 2015
Contact name Maria Cecilia Costa
Organization name Wageningen University
Department Plant Physiology
Lab Wageningen Seed Lab
Street address Droevendaalsesteeg, 1
City Wageningen
State/province The Netherlands
ZIP/Postal code 6708PB
Country Netherlands
Platforms (1)
GPL19371 [AraGene-1_1-st] Arabidopsis Gene 1.1 ST Array [CDF: aragene11st_At_TAIRT.cdf version 17]
Samples (15)
GSM1535440 0h incubation, biological rep1
GSM1535441 0h incubation, biological rep2
GSM1535442 0h incubation, biological rep3
BioProject PRJNA265873

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Supplementary file Size Download File type/resource
GSE62876_RAW.tar 93.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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