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| Status |
Public on Jun 05, 2015 |
| Title |
Regulatory RNA Apela Is a Component of a Negative Feedback Loop Regulating p53-Mediated Apoptosis in Embryonic Stem Cells (III) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The goal is to identify RNAs that bind to Hnrnpl (hnRNP L) using RNA immunoprecipitation (RIP) assay.
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| Overall design |
p53+/+ mES cells and p53-/- mES cells were untreated or treated with 0.5 uM Adriamycin for 8 hours. RNA immunoprecipitation was performed using Hnrnpl antibody (Bethyl, Cat#: A311-423A). Total RNA was extracted and subject to RNAseq.
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| Contributor(s) |
Huang J |
| Citation(s) |
25936916 |
| Submission date |
Oct 15, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jing Huang |
| E-mail(s) |
huangj3@mail.nih.gov
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| Organization name |
National Cancer Institute
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| Lab |
Cancer Biology and Genetics
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| Street address |
37 Convent Dr. 37/3140
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
MD 20892 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| GSM1526264 |
p53-/-, Adriamycin, input RS70 |
| GSM1526265 |
p53+/+, untreated, hnRNP L, RIP RS71 |
| GSM1526266 |
p53+/+, Adriamycin, hnRNP L, RIP RS72 |
| GSM1526267 |
p53-/-, untreated, hnRNP L, RIP RS73 |
| GSM1526268 |
p53-/-, Adriamycin, hnRNP L, RIP RS74 |
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| This SubSeries is part of SuperSeries: |
| GSE65491 |
Regulatory RNA Apela Is a Component of a Negative Feedback Loop Regulating p53-Mediated Apoptosis in Embryonic Stem Cells |
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| Relations |
| BioProject |
PRJNA263940 |
| SRA |
SRP048945 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62378_RAW.tar |
19.4 Mb |
(http)(custom) |
TAR (of XLS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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