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| Status |
Public on Aug 14, 2015 |
| Title |
Identification of Lhx5 binding sites |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Lhx5 mutant mouse embryos show loss of a neuronal nucleus of the brain, called the mammillary body and essential for memory. We found out that this is caused by a loss of appropriate specification of mammillary neurons and we identified in a microarray study downstream targets responsible for the observed phenotype. Here we wanted to find out which of our identified targets are under direct control of Lhx5.
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| Overall design |
In order to identify the direct targets of Lhx5 we decided to use a ChIP-Seq approach. For this approach we generated a stably transfected Neuro2a cell line that expresses a FLAG-tagged version of Lhx5 under the control of the Tet-ON system. As a control we transfected Neuro2a cells with a construct that only expresses the FLAG-tag.
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| Contributor(s) |
Heide M, Alvarez-Bolado G |
| Citation(s) |
26321924 |
| Submission date |
Sep 19, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gonzalo Alvarez-Bolado |
| Organization name |
Institute for Anatomy and Cell Biology
|
| Street address |
Im Neuenheimer Feld 307
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE61614 |
Identification of Lhx5 binding sites and Gene expression data from Lhx5 mutant mouse embryos |
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| Relations |
| BioProject |
PRJNA261492 |
| SRA |
SRP047325 |
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